Tween 80的性質(zhì)及實驗室應(yīng)用注意事項
分子式：C64 H124 O26
分子量：1310 g/mol
外表：Amber colored viscous liquid
密度：1.06-1.09 g/mL, oily liquid
學(xué)名：聚氧乙烯山梨糖醇酐單油酸酯，又名T-80乳化劑
別名: Polysorbate 80, PEG (80) sorbitan monooleate, polyoxyethylenesorbitan monooleate
形成微球的分子量: 76 kDa
制備：山梨糖醇酐單油酸酯和氧化乙烯反應(yīng)制得
生物學(xué)實驗中應(yīng)用
TWEEN 80廣泛應(yīng)用于生化實驗當中，包括
①穩(wěn)定蛋白構(gòu)象；
②從培養(yǎng)基培養(yǎng)的細胞中分離細胞核；
③培養(yǎng)桿菌，如分枝桿菌；
④用作乳化劑。
TWEEN 80在冰激淋加工中的作用
Tween 80 is often used in ice cream to prevent milk proteins from completely coating the fat droplets. This allows them to join together in chains and nets, to hold air in the mixture, and provide a firmer texture, holding its shape as the ice cream melts.
用Tween 80配制溶液
TWEEN 80 is miscible in water (0.1 ml/ml) yielding a clear to slightly hazy faint yellow solution. It is reported to be miscible with alcohol, cottonseed oil, corn oil, ethyl acetate, methanol, and toluene, but insoluble in mineral oil.
The pH of a 1% aqueous solution is 5.5-7.2.
穩(wěn)定性及保存
Aqueous solutions of polysorbates as well as the neat liquid will undergo autoxidation over time, with changes being catalyzed by light, increased temperature, and copper sulfate. （Tween 80易發(fā)生自氧化 作用，應(yīng)避光 、低溫保存）
Solutions are reasonably stable at 2 - 8 ℃ for short periods.
For special applications, storage under argon or nitrogen may be preferred.
Sigma的產(chǎn)品Tween 80并非無菌的. Sigma建議用0.22微粒孔徑的濾膜過濾除菌，不建議采用高溫滅菌的方式。
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